张开翔
郑州大学药学院教授，博士生导师，国家优青基金获得者。2017年博士毕业于清华大学化学系。主要研究方向为：1）基于基因编辑工具的体内原位基因信息分析方法研究；2） 基于核酸纳米材料的药物递送和基因治疗。相关成果以第一作者或通讯作者在Chem. Soc. Rev., J. Am. Chem. Soc., Angew. Chem. Int. Ed., Adv. Mater., J. Extracell. Vesicles, Nano Lett., Chem. Sci.等期刊发表论文30余篇, 累计发表SCI收录论文70余篇，被引3400余次。作为项目负责人承担国家自然科学基金项目3项。
联系方式：zhangkx@zzu.edu.cn
工作与教育经历：
2021.10-至今，郑州大学，药学院，教授
2017.7 – 至今 郑州大学 药学院 副教授
2012.8 – 2014.8 美国加州大学尓湾分校 药学院 联合培养博士
2011.9 – 2017.7 清华大学 化学系 博士
2007.9 – 2011.7 同济大学 材料科学与工程系 学士
荣誉称号及奖励：
•2022年河南省高层次人才（C类）
•2020年河南省高校科技创新人才
•2017年清华大学“启航奖”金奖
专著与教材：
• 《Experiments in Analytical Chemistry》，郑州大学出版社，2021年，主编，ISBN:978-7-5645-7617-2
主持或参加研究项目：
1. 国家优秀青年科学基金项目，22122409, CRISPR生物探针构建与生物传感，2022/01-2024/12，在研，主持。
2. 国家自然科学基金联合培育项目，U2004197，外泌体的单颗粒多参数荧光原位成像分析方法设计及应用研究，2021/01-2023/12，在研，主持。
3. 国家自然科学基金青年项目，21904119，近端结合CRISPR探针构建及细胞核基因点突变直接成像研究，2020/01-2022/12，结题，主持。
4. 河南省高校科技创新人才，21HASTIT043，单细胞、单颗粒、单分子分析，2021/01-2023/12，在研，主持。
5. 中原青年拔尖人才，ZYQR201912191，数字化尿液外泌体检测芯片用于膀胱癌早期筛查及耐药性分析，2020/01-2021/12，结题，主持。
6. 河南省科技攻关项目，192102310147，基于酵母天然机制的一步化CRISPR/Cas9纳米药物制备及基因治疗研究，2020/01-2021/12，结题，主持。
学术论文：
第一作者和通讯作者论文：
2022年
1. Zhang, T.#; Deng, R.*#; Wang, Y.#; Wu, C.#; Zhang, K.#; Wang, C.; Gong, N.; Ledesma-Amaro, R.; Teng, X.; Yang, C.; Xue, T.; Zhang, Y.; Hu, Y.; He, Q.; Li, W. *; Li, J*, A paper-based assay for the colorimetric detection of SARS-CoV-2 variants at single-nucleotide resolution. Nat. Biomed. Eng. 2022, 6, 957-967.
2. Shi, J.#; Nie, W.#; Zhao, X.; Yang, X.; Cheng, H.; Zhou, T.; Zhang, Y.*; Zhang, K.*; Liu, J.*, An Intracellular Self-Assembly-Driven Uninterrupted ROS Generator Augments 5-Aminolevulinic-Acid-Based Tumor Therapy. Adv. Mater. 2022, 34, 2201049.
3. Zhang, K.#; Ma, Y.#; Wang, D.; Liu, J.; An, J.; Li, Y.; Ma, C.; Pei, Y.; Zhang, Z.*; Liu J.*; Shi, J.*, In Vivo Activation of T-Cell Proliferation by Regulating Cell Surface Receptor Clustering Using a pH-Driven Interlocked DNA Nano-Spring. Nano Lett. 2022, 22, 1937-1945.
4. Liang, Y.; Wu, S.; Han, W.; Wang, J.; Xu, C.; Shi, J.; Zhang, Z.*; Gao, H.*; Zhang, K.*; Li, J., Visualizing Single-Nucleotide Variations in a Nuclear Genome Using Colocalization of Dual-Engineered CRISPR Probes. Anal. Chem. 2022, 94, 11745-11752.
5. Li, Y.#; Pei, Y.#; Huang, D.; Xu, R.; Wu, Y.; Li, Y.; Zhou, R.; Wei, Y.; Tan, X.; Gao, H.; Shi, J.; Zhang, Z.*; Zhang, K.*; Li, J.*, Direct visualization of living bacterial genotypes using CRISPR/Cas12a-circular reporter nanoprobes. Biosens.. Bioelectron. 2022, 216, 114641.
6. Wang, D.; Liu, J.; Duan, J.; Ma, Y.; Gao, H.; Zhang, Z.; Liu, J.*; Shi, J.*; Zhang, K.*, Photocontrolled Spatiotemporal Delivery of DNA Immunomodulators for Enhancing Membrane-Targeted Tumor Photodynamic Immunotherapy. ACS Appl. Mater. Interfaces. 2022, 14, 44183-44198.
7. Yu, W.; Xuan, C.; Liu, B.; Zhou, L.; Yin, N.; Gong, E.; Zhang, Z., Li, Y.*, Zhang, K.*, Shi, J.*, Carrier-free programmed spherical nucleic acid for effective ischemic stroke therapy via self-delivery antisense oligonucleotide. Nano Res. 2022, 1-11.
8. Yu, W.; Yin, N.; Yang, Y.; Xuan, C.; Liu, X.; Liu, W.; Zhang Z.; Zhang, K.*; Liu, J.*; Shi, J.*, Rescuing ischemic stroke by biomimetic nanovesicles through accelerated thrombolysis and sequential ischemia-reperfusion protection. Acta Biomater. 2022, 140, 625-640.
2021年
9. Shi, J.#; Yang, Y.#; Yin, N.; Liu, C.; Zhao, Y.; Cheng, H.; Zhou, T.; Zhang, Z.*; Zhang, K.*, Engineering CXCL12 Biomimetic Decoy‐Integrated Versatile Immunosuppressive Nanoparticle for Ischemic Stroke Therapy with Management of Overactivated Brain Immune Microenvironment. Small Methods. 2021, 6, e2101158.
10. Shi, J.#; Wang, D.#; Ma, Y.; Liu, J.; Li, Y.; Reza, R.; Zhang, Z.; Liu, J.*; Zhang, K.*, Photoactivated Self-Disassembly of Multifunctional DNA Nanoflower Enables Amplified Autophagy Suppression for Low-Dose Photodynamic Therapy. Small. 2021, 17, e2104722.
11. Liu, J.#; Yu, W.#; Han, M.; Liu, W.; Zhang, Z.*; Zhang, K.*; Shi, J.*, A specific “switch-on” type magnetic resonance nanoprobe with distance-dominate property for high-resolution imaging of tumors. Chem. Eng. J. 2021, 404, 126496.
12. Liu, J.#; Jin, Y.#; Song, Z.; Xu, L.; Yang, Y.; Zhao, X.; Wang, B.; Liu, W.; Zhang, K.*; Zhang, Z.*; Shi, J.*, Boosting tumor treatment by dredging the hurdles of chemodynamic therapy synergistic ion therapy. Chem. Eng. J. 2021, 411.
13. Li, A.#; Liang, C.#; Xu, L.; Wang, Y.; Liu, W.; Zhang, K.*; Liu, J.*; Shi, J.*, Boosting 5-ALA-based photodynamic therapy by a liposomal nanomedicine through intracellular iron ion regulation. Acta Pharm. Sin. B 2021, 11, 1329-1340.
14. Liu, J.#; Zhao, X.#; Nie, W.; Yang, Y.; Wu, C.; Liu, W.; Zhang, K.*; Zhang, Z.*; Shi, J.*, Tumor cell-activated “Sustainable ROS Generator” with homogeneous intratumoral distribution property for improved anti-tumor therapy. Theranostics 2021, 11, 379-396.
15. Guo, Y.*; Wu, D.; Zhang, X.; Zhang, K.*; Luo, Y.*, Biomolecules in cell-derived extracellular vesicle chariots as warriors to repair damaged tissues. Nanoscale 2021, 13, 16017-16033.
2020年
16. Zhang, K.; Deng, R.; Gao, H.; Teng, X.; Li, J.*, Lighting up single-nucleotide variation in situ in single cells and tissues. Chem. Soc. Rev. 2020, 49, 1932-1954.
17. Zhang, J.#; Shi, J.#; Zhang, H.; Zhu, Y.; Liu, W.; Zhang, K.*; Zhang, Z.*, Localized fluorescent imaging of multiple proteins on individual extracellular vesicles using rolling circle amplification for cancer diagnosis. J. Extracell. Vesicles 2020, 10, e12025.
18. Liu, J.#; Zhu, C.#; Xu, L.; Wang, D.; Liu, W.; Zhang, K.*; Zhang, Z.*; Shi, J.*, Nanoenabled Intracellular Calcium Bursting for Safe and Efficient Reversal of Drug Resistance in Tumor Cells. Nano Lett. 2020, 20, 8102-8111.
19. Shi, J.; Yu, W.; Xu, L.; Yin, N.; Liu, W.; Zhang, K.*; Liu, J.*; Zhang, Z.*, Bioinspired Nanosponge for Salvaging Ischemic Stroke via Free Radical Scavenging and Self-Adapted Oxygen Regulating. Nano Lett. 2020, 20, 780-789.
20. Zhang, K.#; Qin, S.#; Wu, S.; Liang, Y.; Li, J.*, Microfluidic systems for rapid antibiotic susceptibility tests (ASTs) at the single-cell level. Chem. Sci. 2020, 11, 6352-6361.
21. Zhang, K.#; Li, Y.#; Liu, J.; Yang, X.; Xu, Y.; Shi, J.*; Liu, W.*; Li, J.*, Y-Shaped Circular Aptamer–DNAzyme Conjugates for Highly Efficient in Vivo Gene Silencing. CCS Chemistry 2020, 2, 631-641.
22. Shi, J.#; Yang, X.#; Li, Y.; Wang, D.; Liu, W.; Zhang, Z.; Liu, J.*; Zhang, K.*, MicroRNA-responsive release of Cas9/sgRNA from DNA nanoflower for cytosolic protein delivery and enhanced genome editing. Biomaterials 2020, 256, 120221.
23. Yan, Y.#; Zhang, K.#; Wang, H.; Liu, W.; Zhang, Z.; Liu, J.*; Shi, J.*, A fullerene based hybrid nanoparticle facilitates enhanced photodynamic therapy via changing light source and oxygen consumption. Colloids Surf. B. Biointerfaces 2020, 186, 110700.
24. Zhang, K.; Liu, J.; Song, Q.; Wang, D.; Shi, J.*; Zhang, H.*; Li, J.*, Multifunctional DNA Nanoflowers for Autophagy Inhibition and Enhanced Antitumor Chemotherapy. Chemical Journal of Chinese Universities-Chinese 2020, 41, 1461-1469.
2019年
25. Zhang, K.; Gao, H.; Deng, R.; Li, J.* Emerging Applications of Nanotechnology for Controlling Cell-Surface Receptor Clustering. Angew. Chem. Int. Ed. Engl. 2019, 58, 4790-4799.
26. Zhang, K.#; Liu, J.#; Song, Q.; Yang, X.; Wang, D.; Liu, W.; Shi, J.*; Zhang, Z.* DNA Nanosponge for Adsorption and Clearance of Intracellular miR-21 and Enhanced Antitumor Chemotherapy. ACS Appl. Mater. Interfaces. 2019, 11, 46604-46613.
27. Gao, H.; Zhang, K.*; Teng, X.; Li, J.* Rolling circle amplification for single cell analysis and in situ sequencing. TrAC, Trends Anal. Chem. 2019, 121, 115700.
28. Zhang, K.#; Yue, Y.#; Wu, S.; Liu, W.*; Shi, J.*; Zhang, Z.* Rapid Capture and Non-destructive Release of Extracellular Vesicles using Aptamer-based Magnetic Isolation. ACS Sens. 2019, 4, 1245-1251.
29. Liu, J.#; Zhang, Y.#; Liu, W.; Zhang, K.*; Shi, J.*; Zhang, Z.* Tumor Antigen Mediated Conformational Changes of Nanoplatform for Activated Photodynamic Therapy. Adv. Healthc. Mater. 2019, e1900791.
30. Liu, J.; Liu, W.; Zhang, K.*; Shi, J.*; Zhang, Z.* A Magnetic Drug Delivery System with "OFF-ON" State via Specific Molecular Recognition and Conformational Changes for Precise Tumor Therapy. Adv. Healthc. Mater. 2019, e1901316.
31. Li, Y.; Zhai, Y.; Liu, W.; Zhang, K.*; Liu, J.*; Shi, J.*; Zhang, Z., Ultrasmall nanostructured drug based pH-sensitive liposome for effective treatment of drug-resistant tumor. J Nanobiotechnology 2019, 17, 117.
32. Liu, W.#; Zhang, K.#; Zhuang, L.; Liu, J.; Zeng, W.; Shi, J.*; Zhang, Z.*, Aptamer/photosensitizer hybridized mesoporous MnO(2) based tumor cell activated ROS regulator for precise photodynamic therapy of breast cancer. Colloids Surf. B. Biointerfaces 2019, 184, 110536.
2015年-2018年
33. Zhang, K.; Deng, R.; Teng, X.; Li, Y.; Sun, Y.; Ren, X.; Li, J.* Direct Visualization of Single-Nucleotide Variation in mtDNA Using a CRISPR/Cas9-Mediated Proximity Ligation Assay. J. Am. Chem. Soc. 2018, 140, 11293-11301.
34. Zhang, K.; Deng, R.; Sun, Y.; Zhang, L.; Li, J.* Reversible control of cell membrane receptor function using DNA nano-spring multivalent ligands. Chem. Sci. 2017, 8, 7098-7105.
35. Zhang, K.; Deng, R.; Li, Y.; Zhang, L.; Li, J.* Cas9 cleavage assay for pre-screening of sgRNAs using nicking triggered isothermal amplification. Chem. Sci. 2016, 7, 4951-4957.
36. Zhang, K.; Kang, D. K.; Ali, M. M.; Liu, L.; Labanieh, L.; Lu, M.; Riazifar, H.; Nguyen, T. N.; Zell, J. A.; Digman, M. A.; Gratton, E.; Li, J.*; Zhao, W.* Digital quantification of miRNA directly in plasma using integrated comprehensive droplet digital detection. Lab Chip. 2015, 15, 4217-4226.
