Determination of 2-methoxyestradiol in serum samples and pharmaceutical preparations by silver nanoparticles-enhanced chemiluminescence

【作者】 Min Zhang, XiangqinXiao, WenyuanZeng, XiaoyingZeng, HanchunYao

【期刊名】Talanta

【影响因子】 2014年: 3.545

【作者单位】 School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, PR China

【年，卷(期)：页码】2014, 120: 331-335

【关键词】Silver nanoparticles; Chemiluminescence; 2-methoxyestradiol; Serum samples

【摘要】Silver nanoparticles (AgNPs) exhibited better chemiluminescence (CL) catalysis activity and smaller nanoparticles have stronger catalysis ability in luminol–K3Fe(CN)6 system among the synthesized AgNPs of different size.10±2 nm nanoparticles was used as catalysts to enhance the reaction sensitivity. It was found that the CL intensity of AgNPs–luminol–K3Fe(CN)6 was strongly inhibited in the presence of 2-methoxyestradiol (2-ME) and the relative CL intensity was in linear correlation with the concentration of 2-ME. Thus, the silver nanoparticles-enhanced CL method for the determination of 2-ME was developed. The proposed method has a detection limit (3 Sb/K) of 5.0×10

^-10 mol L

1 with arelative standard deviationof 0.75% for 5.0×10

^-8 mol L

1 2-ME. The method was successfully applied for determination of 2-ME in human serum and pharmaceutical preparations. The possible CL reaction mechanism was also discussed briefly. Oxygen radicals played an mportant role in the catalytic process.

【全文链接】

http://dx.doi.org/10.1016/j.talanta.2013.11.048