A “submunition” dual-drug system based on smart hollow NaYF4/apoferritin nanocage for upconversion imaging
【作者】 Jie Zhou, Shanshan Chen, Chong Sun, Qiuzheng Du, Pei Luo, Bin Du and Hanchun Yao
【期刊名】 RSC Advances
【影响因子】 2017年: 3.289
【作者单位】School of Pharmacy, Zhengzhou University, Zhengzhou, Henan 450001, P.R. China
【年，卷(期)：页码】2016, 6, 33443–33454
【关键词】 Controlled drug release; NaYF4; Tumor-targeting; upconversion imaging
【摘要】Synergetic therapy has exhibited important potential for the treatment of cancers, especially for drugresistant cancers. In this report, bifunctional nanomaterials based on doxorubicin (DOX)-loaded NaYF4 and verapamil (Vp)-loaded apoferritin nanocage dual-drug system (DOX/NaYF4-Vp/AFn-FA) weresynthesized for in vivo upconversion imaging and enhanced chemotherapy in breast cancers. Moreover, folic acid (FA) targeting promoted the cellular uptake, and accelerated the release of DOX in drugsensitive MCF-7. This system is a multifunctional drug delivery system with significant tumor-targeting efficacy and is also the first time preparation of NaYF4–AFn-FA. The dual-drug system enabled a temporal release of two drugs: Vp was released rapidly to inhibit the activity of P-gp and restore cell apoptotic signaling pathways, while DOX was released in a more sustained manner and highly accumulated in drug resistant cells to exert a therapeutic effect, due to the inactivation of P-gp by Vp. Toxicity assessment in vitro and in vivo revealed the good biocompatibility of the as-prepared DOX/ NaYF4-Vp/AFn-FA nanocomposites. In addition, NaYF4–AFn-FA uptaken by cells and mouse by intravenous injection showed bright green emission without background noise under 980 nm infrared laser excitation. Thus, NaYF4–AFn-FA has the potential for simultaneous targeted anti-cancer drug delivery or imaging, which suggested a new multi-mechanism pathway for tumor treatment.
【全文链接】http://pubs.rsc.org/en/content/articlelanding/2016/ra/c5ra24285a#!divAbstract
