Flavokawain A inhibits prostate cancer cells by inducing cell cycle

arrest and cell apoptosis and regulating the glutamine metabolism

Pathway

【作者】Kaili Wang, Weijie Zhang, Zihan Wang, Ming Gao, Xinying Wang, Wenchao Han, Nan Zhang, Xia Xu

【期刊名】Journal of Pharmaceutical and Biomedical Analysis

【影响因子】 2019年:3.209

【作者单位】School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, PR China

【年，卷：页码】2020, 186 : 113288

【关键词】Flavokawain A, Prostate cancer, Cell cycle, Glutamine metabolism, Cell apoptosis, Metabolomics

【摘要】Flavokawain A (FKA), a major chalcone in kava extracts, has exhibited anti-proliferative and apoptotic effects in the prostate cancer. However, the molecular mechanism of FKA remains unclear. In this study, FKA induces cell apoptosis and cell cycle arrest in a G2M phase to prostate cancer cells. FKA interferes with tubulin polymerization and inhibits survivin expression in PC3 cells. Molecular docking simulation experiment fifinds that FKA can bind to colchicine binding sites that inhibit tubulin polymerization. FKA treatment regulates the glutamine metabolism pathway in PC3 cells by reducing intracellular glutamine,

glutamic and proline. FKA treatment also decreases the GSH content by decreasing the activity of GSH synthetase (GSS) and increasing the activity of glutathione thiol transferase (GSTP1), which subsequently

induces ROS production and PC3 cell apoptosis.

【全文链接】https://doi.org/10.1016/j.jpba.2020.113288