基本信息：
许红恩，男，副教授，硕士生导师
地址：郑州市大学路40号
邮箱：hongen_xu@zzu.edu.cn
邮编：450052
学习及工作经历：
2023年至今：郑州大学医学科学院精准医学中心副教授
2018年-2022年：郑州大学医学科学院精准医学中心讲师，拔尖博士
2012年-2017年：德国慕尼黑工业大学生物信息学系生物信息学博士
2009年-2012年：西南大学蚕学与系统生物学研究所生化与分子硕士（免试）
2005年-2009年：西南大学生物技术学士
研究方向：
（1）遗传性耳聋分子诊断及致病机制研究；
（2）遗传性肾病分子诊断；
（3）基于深度突变扫描技术的基因变异功能/靶向药物敏感性高通量研究；
专利：
1.一种高通量突变体文库的构建方法，专利号：ZL202110098177.X，排名第四
2.人类EGFR基因错义突变分子标志物及其在预测靶向抑制剂抗药性中的应用，专利号：ZL202110745168.5，排名第二
3.二代DNA测序样本的特异性标签防错试剂盒，专利号：ZL201911383583.X，排名第三
4.一种基于多重PCR及高通量测序技术检测耳聋基因致病变异的特异性引物、试剂盒及应用，专利号：ZL202010992539.5，排名第二
5.一种应用于检测药物性耳聋试剂的碳点及其制备方法，专利号：ZL 2021 1 0642054.8，排名第四
6.SELENOO基因突变体及其应用，专利号：ZL 2023 1 0039393.6，排名第一
室间质量评价证书：
1.2018年，卫生部临床检验中心“遗传病高通量测序检测生信分析室间质评”；
2.2019年，国家卫健委临检中心“全国遗传变异解读及报告质量评价”；
3.2020年，国家卫健委临检中心“全国新生儿耳聋基因检测室间质量评价”；
4.2021年，国家卫健委临检中心“全国胚系基因变异全外显子测序检测室间质量评价”
学术兼职：
医学检验质量管理专业委员会遗传与出生缺陷防控学组委员
上海遗传学会临床遗传与遗传咨询专业委员会高级会员
河南省预防医学会出生缺陷防治专业委员会常务委员
所获荣誉：
郑州大学科技服务社会先进个人（2021年）
中国康复医学会科技进步一等奖（2023年），卢伟;许红恩;孙淑萍;张金慧;薛夏;陈蓓;蓝天翔;周慧芳;银力;蓝军;汤文学，听力障碍的精准诊断和全生命周期听力康复体系的构建和推广
研究生：
联合指导研究生21名：
2018级：田永安、张森、张静、潘昭宇、李金映
2019级：曾焙枰、田均波、冯海锋
2020级：胡桑、许世成、王璐璐、张文元
2021级：赵月、张腾、何万里
2022级：连成玉、王韩君、李新宇
2023级：张婷、马秉彤、王雨思
独立指导研究生3名：
2021级：毛璐
2022级：刘梦丽
2023级：张梦丽
主持或主要参与项目：
1.国家自然科学基金面上项目，GJB6基因R75W突变导致显性遗传性耳聋的致病机制研究，82371160，2024/01-2027/12，49万，参与
2.国家自然科学基金青年科学基金项目，基于深度突变扫描技术的遗传性耳聋GJB2基因错义变异功能研究，82101963，2022/01-2024/12，30万，主持
3.河南省重点研发与推广专项（科技攻关），基于外显子组测序重构转移和复发性骨肉瘤克隆群体结构，192102310034，2019/01-2020/12，10万，主持
4.河南省高等学校重点科研项目计划，基于外显子组测序的骨肉瘤化疗耐药相关基因及突变初探，19B310007，2019/01-2020/12，指导计划，主持
5.郑州大学青年教师科研启动项目（拔尖博士），2018-2020，20万，主持
6.国家卫生健康委出生缺陷预防重点实验室开放课题，遗传性耳聋致病基因GJB2变异功能的高通量研究，ZD202007，2020/08-2021/12，8万，主持
7.河南省医学科技攻关计划联合共建项目，遗传性耳聋二代测序数据一站式分析流程及变异数据库的搭建，LHGJ20190317，5万，主持
发表论文：
#共同一作，*通讯作者
1.左彬,王璐璐,毛璐,许红恩,孙淑萍,卢伟. Heimler综合征表型及致病变异分析1例.中华耳鼻咽喉头颈外科杂志, 2024, 59(3): 249-252.
2.Shelby E. Redfield#, Pedro De-la-Torre#, Mina Zamani§, Hanjun Wang§, Hina Khan, Tyler Morris, Gholamreza Shariati, Majid Karimi, Margaret A. Kenna, Go Hun Seo,Hongen Xu, Wei Lu, Sadaf Naz, Hamid Galehdari, Artur A. Indzhykulian*, A. Eliot Shearer* & Barbara Vona*. PKHD1L1, a gene involved in the stereocilia coat, causes autosomal recessive nonsyndromic hearing loss. Hum. Genet. (2024). (§共同二作)
3.雷一波,孙淑萍,毛璐,许红恩,汤文学,潘昭宇,卢伟. LARS2和HARS2基因致病变异所致的Perrault综合征分析.中华耳鼻咽喉头颈外科杂志, 2023, 58(12):1191-1197.
4.何万里,许红恩,刘梦丽,张腾,卢伟.两个鳃耳综合征家系的临床表型及致病变异分析.听力学及言语疾病杂志, 2023,31:1-5
5.Qian Dou,Hongen Xu, Liying Ma, Li Tan*, Wenxue Tang*. Phenotypic variability in two female siblings with oocyte maturation arrest due to a TUBB8 variant. BMC Medical Genomics, 2023,16:271.
6.张文元#,毛璐#,张金慧,许红恩,陈蓓.多发性骨性连接综合征１型一个家系的表型及致病变异分析.中华医学遗传学杂志, 2023, 40(9):57-62.
7.王璐璐,毛璐,许红恩,孙淑萍,左彬,卢伟. Waardenburg综合征4个家系的临床表型与基因变异分析.中华医学遗传学杂志, 2023, 40(6):661-667.
8.文龙,刘福云,段世超,许红恩,夏冰,董延召,程文哲,胡伟明,冯国明,张晴. GLI3：c.2880del导致的多指(趾)家系分析.中华小儿外科杂志, 2023, 44(5):427-433.
9.Lu Mao, Yueqiang Wang, Lei An, Beiping Zeng, Yanyan Wang , Dmitrij Frishman, Mengli Liu,Yanyu Chen, Wenxue Tang,Hongen Xu*. Molecular Mechanisms and Clinical Phenotypes of GJB2 Missense Variants.Biology2023, 12(4):505.
10.许红恩,袁永一.中美遗传性耳聋病因诊断指南对比解读.中华耳鼻咽喉头颈外科杂志, 2023, 58(4):416-418.
11.中国耳聋基因筛查与诊断临床多中心研究协作组,中华耳鼻咽喉头颈外科杂志编辑委员会,中华医学会耳鼻咽喉头颈外科学分会袁永一,黄莎莎,王国建，韩明昱,戴朴,许红恩,卢宇,程静,高雪,马璐,刘玉和,杨涛.中华耳鼻咽喉头颈外科杂志, 2023, 58(1):3-14
12.曾焙枰#,许红恩#,毛璐,汤文学.遗传性耳聋分子诊断及梯级检测策略应用.遗传, 2023, 45(1):29-41.
13.Lei An#, Yueqiang Wang#, Guangyao Wu, Zhenxing Wang, Zeyuan Shi, Chang Liu, Chunli Wang, Ming Yi, Chenguang Niu, Shaofeng Duan, Xiaodong Li, Wenxue Tang, Kongming Wu, Shuqing Chen*,Hongen Xu*. Defining the sensitivity landscape of EGFR variants to tyrosine kinase inhibitors.Translational Research. 2023, 255:14-25.
14.冯海锋#,许红恩#,陈蓓,孙淑萍,曾焙枰,汤文学,卢伟*. 5个鳃耳（肾）综合征家系的致病突变和诊疗分析.中华耳鼻咽喉头颈外科杂志, 2022, 57(12):1433-1441
15.Bin Zuo#,Hongen Xu#, Zhaoyu Pan, Lu Mao, Haifeng Feng, Beiping Zeng, Wenxue Tang*, Wei Lu*. A likely pathogenic POLD1 variant associated with mandibular hypoplasia, deafness, progeroid features, and lipodystrophy syndrome in a Chinese patient.BMC Medical Genomics. 2022, 15:220.
16.Lan Jiang,Hongen Xu, Danhua Liu, Sen Zhang, Ying Xu*. Case report: Clinical and genetic analysis of a family with nonsyndromic auditory neuropathy.Frontiers in Pediatrics. 2022,10:1005335.
17.Beiping Zeng#,Hongen Xu#, Yanan Yu, Siqi Li, Yongan Tian, Tiandong Li, Zengguang Yang, Haili Wang, Guangke Wang, Mingxiu Chang*, Wenxue Tang*. Increased diagnostic yield in a cohort of hearing loss families using a comprehensive stepwise strategy of molecular testing.Frontiers in Genetics, 2022,13:1057293.
18.Zhigang Wang#*, Yuanbo Qi#, Yonghua Feng#,Hongen Xu, Junxiang Wang, Luyu Zhang, Jie Zhang, Xinyue Hou, Guiwen Feng*, Wenjun Shang*. The N6-methyladenosine writer WTAP contributes to the induction of immune tolerance post kidney transplantation by targeting regulatory T cells.Laboratory Investigation. 2022,102(11):1268-1279.
19.Junbo Tian#, Jing Zhang#, Zengguang Yang, Shuaisheng Feng, Shujuan Li, Shiqi Ren, Jianxiang Shi, Xinyue Hou, Xia Xue, Bei Yang,Hongen Xu*, Jiancheng Guo*. Genetic Epidemiology of Medication Safety and Efficacy Related Variants in the Central Han Chinese Population With Whole Genome Sequencing.Frontier in Pharmacology. 2022, 12:790832.
20.Beiping Zeng#,Hongen Xu#, Yongan Tian, Qianyu Lin, Haifeng Feng, Zhifeng Zhang, Siqi Li, Wenxue Tang*. A novel splicing variant in the TMC1 gene causes non-syndromic hearing loss in a Chinese family.Chinese Medical Journal. 2022, 135(21):2631-2633.
21.Alec Steep, Evin Hildebrandt,Hongen Xu, Cari Hearn, Dmitrij Frishman, Masahiro Niikura, John R. Dunn, Taejoong Kim, Steven J. Conrad, William M. Muir and Hans H. Cheng*. Identification and Validation of Ikaros (IKZF1) as a Cancer Driver Gene for Marek’s Disease Virus-Induced Lymphomas.Microorganisms. 2022, 135(21):2631-2633.
22.Xiaoxuan Wang#, Sen Zhang#, Sang Hu,Hongen Xu, Qi Zhang*, Wenxue Tang*. Establishment of an induced pluripotent stem cell line (ZZUPMCi001-A) derived from peripheral blood mononuclear cells in a patient with type 1 Waardenburg syndrome.Stem Cell Research. 2021,57:102606.
23.Haifeng Feng#,Hongen Xu#, Bei Chen#, Shuping Sun, Rongqun Zhai, Beiping Zeng, Wenxue Tang*, Wei Lu*. Genetic and Phenotypic Variability in Chinese Patients With Branchio-Oto-Renal or Branchio-Oto Syndrome.Frontiers in Genetics, 2021, 12:765433.
24.Zhi-Gang Wang#*,Hong-En Xu#, Fu-Min Cheng#, Jie Zhang, Yong-Hua Feng, Dan-Hua Liu, Wen-Jun Shang*, Gui-Wen Feng*. Donor BMSC-derived small extracellular vesicles relieve acute rejection post-renal allograft through transmitting Loc108349490 to dendritic cells.Aging Cell, 2021, 20(10):e13461.
25.Zhigang Wang#,Hongen Xu#, Tianchao Xiang#, Danhua Liu, Fei Xu, Lixiang Zhao, Yonghua Feng, Linan Xu, Jialu Liu, Ye Fang, Huanfei Liu, Ruijun Li, Xinxin Hu, Jingyuan Guan, Longshan Liu, Guiwen Feng, Qian Shen, Hong Xu, Dmitrij Frishman, Wenxue Tang, Jiancheng Guo*, Jia Rao*, Wenjun Shang*. An accessible insight into genetic findings fortransplantation recipients with suspected genetickidney disease.Npj Genomic Medicine, 2021, 6(1):57.
26.Yongan Tian#,Hongen Xu#, Danhua Liu, Juanli Zhang, Zengguang Yang, Sen Zhang, Huanfei Liu, Ruijun Li, Yingtao Tian, Beiping Zeng, Tong Li, Qianyu Lin, Haili Wang, Xiaohua Li, Wei Lu, Ying Shi, Yan Zhang, Hui Zhang, Chang Jiang, Ying Xu, Bei Chen*, Jun Liu*, Wenxue Tang*. Increased diagnosis of enlarged vestibular aqueduct by multiplex PCR enrichment and next‐generation sequencing of the SLC26A4 gene.Molecular Genetics & Genomic Medicine, 2021, 9(8):e1734.
27.Sen Zhang#,Hongen Xu#, Yongan Tian, Danhua Liu, Xinyue Hou, Beiping Zeng, Bei Chen, Huanfei Liu, Ruijun Li, Xiaohua Li, Bin Zuo, Ryan Tang, Wenxue Tang*. High genetic heterogeneity in Chinese patients with Waardenburg syndrome revealed by next-generation sequencing.Frontiers in Genetics, 2021, 12:643546.
28.Jinying Li#,Hongen Xu#, Jianfeng Sun, Yongan Tian, Danhua Liu, Yaping Qin, Huanfei Liu, Ruijun Li, Lingling Neng, Xiaohua Deng, Binbin Xue, Changyun Yu*, Wenxue Tang*. Missense Variant of Endoplasmic Reticulum Region of WFS1 Gene Causes Autosomal Dominant Hearing Loss without Syndromic Phenotype.BioMed Research International, 2021, 6624744.
29.Xia Xue#, Jianxiang Shi#,Hongen Xu#, Yaping Qin, Zengguang Yang, Shuaisheng Feng, Danhua Liu, Liguo Jian, Linlin Hua, Yaohe Wang, Qi Zhang, Xueyong Huang, Xiaoju Zhang, Xinxin Li, Chunguang Chen, Jiancheng Guo*, Wenxue Tang*, Jianbo Liu*. Dynamics of binding ability prediction between spike protein and human ACE2 reveals the adaptive strategy of SARS-CoV-2 in humans.Scientific Reports, 2021,11(1):3187.
30.Yingyuan Guo, Yanru Hao, Dejun Zhang,Hongen Xu, Duojiao Yu, Jingmao Lv, Zeming Fu, Shuang Han, Fang Guo, Jie Bai, Guofang Guan*. A novel missense mutation in SMPX causes a rare form of X-linked postlingual sensorineural hearing loss in a Chinese family.Translational Pediatrics, 2021,10(2):2224-4344.
31.Rongqun Zhai#, Haifeng Feng#, Qingli Li, Wei Lu, Danhua Liu, Yongan Tian, Huanfei Liu, Ruijun Li, Bin Zuo, Wenxue Tang,Hongen Xu*, Bei Chen*. Auditory Neuropathy Spectrum Disorder (ANSD)—Clinical Characteristics and Pathogenic Variant Analysis of Three Nonsyndromic Deafness Families.BioMed Research International, 2020, 8843539.
32.Zhaoyu Pan#,Hongen Xu#, Bei Chen#, Yongan Tian, Linlin Zhang, Sen Zhang, Danhua Liu, Huanfei Liu, Ruijun Li, Xinxin Hu, Jingyuan Guan, Wenxu Tang*, Wei Lu*. Treacher Collins syndrome: Clinical report and retrospective analysis of Chinese patients.Molecular Genetics & Genomic Medicine, 2020, 9(2):e1573.
33.Yanfang Mi#, Danhua Liu#, Beiping Zeng, Yongan Tian, Hui Zhang, Bei Chen, Juanli Zhang, Hong Xue, Wenxue Tang, Yulin Zhao*,Hongen Xu*. Early truncation of the N-terminal variable region of EYA4 gene causes dominant hearing loss without cardiac phenotype.Molecular Genetics & Genomic Medicine, 2020, 9(1):e1569.
34.陈蓓#,张森#,田永安,刘欢飞,柳丹华,薛夏,李瑞君,胡欣欣,关景元,汤文学,许红恩*.新的CDH23基因复合杂合模式导致的综合征型耳聋家系研究.中华耳鼻咽喉头颈外科杂志, 2020, 55(9): 822-829
35.Bei Chen#,Hongen Xu#, Yanfang Mi#, Wei Jiang, Dan Guo, Jinhui Zhang, Yulin Zhao, and Wenxue Tang*. Mechanisms of hearing loss and cell death in the cochlea of connexin mutant mice.American Journal of Physiology-Cell Physiology, 2020, 319(3):C569-C578.
36.Zhaoyu Pan#,Hongen Xu#, Yongan Tian, Danhua Liu, Huanfei Liu, Ruijun Li, Qian Dou, Bin Zuo, Ronqun Zhai, Wenxue Tang*, Wei Lu*. Perrault syndrome: Clinical report and retrospective analysis.Molecular Genetics & Genomic Medicine, 2020, 8(10):e1445.
37.Wei Cao*#, Hayan Lee#, Wei Wu*#, Aubhishek Zaman#, Sean McCorkle, Ming Yan, Justin Chen, Qinghe Xing, Nasa Sinnott-Armstrong,Hongen Xu, M Reza Sailani, Wenxue Tang, Yuanbo Cui, Hongyan Guan, Pengju Lv, Xiaoyan Sun, Lei Sun, Pengli Han, Yanan Lou, Jing Chang, Jinwu Wang, Yuchi Gao, Jiancheng Guo, Gundolf Schenk, Alan Hunter Shain, Fred G Biddle, Eric Collisson, Michael Snyder*, Trever G Bivona*. Multi-faceted epigenetic dysregulation of gene expression promotes esophageal squamous cell carcinoma.Nature Communications, 2020, 11(1), 1-19
38.Junkui Sun,Hongen Xu, Muge Qi, Chi Zhang*, Jianxiang Shi*. Identification of key genes in osteosarcoma by meta‑analysis of gene expression microarray.Molecular Medicine Reports, 2019, 20(4), 3075-3084.
39.Dingkang Xu,Hongen Xu, Fang Wang, Guoqing Wang, Qingjie Wei, Shixiong Lei, Qiang Gao, Qi Zhang*, Fuyou Guo*. Multiple Distinctive Demyelinating Lesions Caused by Eosinophilic Granulomatosis With Polyangiitis: Case Report and Literature Review.Frontiers in Neurology, 2019,10, 213.
40.Karolina Rutkowska,Hongen Xu, Krzysztof Flisikowski. Differentially methylated region in bovine MIMT1 detected by small-scale whole-genome methylation sequencing.Journal of Applied Genetics, 2019, 60(3),401-404.
41.Minjin Han#,Hongen Xu#, Xiaomin Xiong, Huahao Zhang*. Evolutionary dynamics of transposable elements during silkworm domestication.Genes & Genomics, 2018, 40, 1041–1051.
42.Feng Shao, Jianrong Wang,Hongen Xu, Zuogang Peng*. FishTEDB: a collective database of transposable elements identified in the complete genomes of fish.Database, 2018, bax106.
43.Jan Smida#*,Hongen Xu#, Yanping Zhang#, Daniel Baumhoer, Sebastian Ribi, Michal Kovac, Irene von Luettichau, Stefan Bielack, Valerie B. O’Leary, Christine Leib-Mösch, Dmitrij Frishman*, and Michaela Nathrath. Genome-wide analysis of somatic copy number alterations and chromosomal breakages in osteosarcoma.International Journal of Cancer, 2017, 141, 816-828. (Frishman, D. and Nathrath, M. shared senior authorship)
44.Hongen Xu, Hubert Pausch, Heli Venhoranta, Karolina Rutkowska, Christine Wurmser, Beate Rieblinger, Tatiana Flisikowska, Dmitrij Frishman, Lech Zwierzchowski, Ruedi Fries, Magnus Andersson, Alexander Kind, Angelika Schnieke, and Krzysztof Flisikowski*. Maternal placenta modulates a deleterious fetal mutation.Biology of Reproduction, 2017, 2, 249-257.
45.Tatiana Flisikowska, Monika Stachowiak,Hongen Xu, Alexandra Wagner, Alejandra Hernandez-Caceres, Christine Wurmser, Carolin Perleberg, Hubert Pausch, Anna Perkowska, Konrad Fischer, Dmitrij Frishman, Ruedi Fries, Marek Switonski, Alexander Kind, Dieter Saur, Angelika Schnieke & Krzysztof Flisikowski*. Porcine FAP model enables systematic analysis of early events in adenoma progression.Scientific Reports, 2017, 7, 6613.
46.Yanping Zhang#,Hongen Xu#, and Dmitrij Frishman*. Genomic determinants of somatic copy number alterations across human cancers.Human Molecular Genetics, 2016, 5, 1019-1030.
47.Minjin Han,Hongen Xu, Huahao Zhang, Cedric Feschotte, and Ze Zhang*. Spy: a new group of eukaryotic DNA transposons without target site duplications.Genome Biology and Evolution, 2014, 6, 1748-1757.
48.Huahao Zhang,Hongen Xu, Yihong Shen, Minjin Han, Ze Zhang*. The Origin and Evolution of Six Miniature Inverted-Repeat Transposable Elements in Bombyx mori and Rhodnius prolixus.Genome Biol Evol, 2013, 5 (11), 2020-2031.
49.Huahao Zhang, Yihong Shen,Hongen Xu, Hongyu Liang, Minjin Han, and Ze Zhang*. A novel hAT element inBombyx moriandRhodnius prolixus: its relationship with miniature inverted repeat transposable elements (MITEs) and horizontal transfer.Insect Mol Biol, 2013, 22, 584-596.
50.Hongen Xu, Huahao Zhang, Tian Xia, Minjin Han, Yihong Shen, and Ze Zhang*. BmTEdb: a collective database of transposable elements in the silkworm genome.Database, 2013, bat055.
51.许红恩，张化浩，韩民锦，沈以红，黄先智，向仲怀，张泽*.真核生物转座子鉴定和分类计算方法.遗传, 2012, 34, 1009-1019.
52.许红恩，韩民锦，张化浩，沈以红，向仲怀，张泽*.家蚕LTR逆转录转座子的鉴定、分类及系统发育分析.昆虫学报, 2011, 54, 1211-1222.《昆虫学报》2014年单篇论文全文pdf最高下载
53.Hongsong Yu, Yihong Shen, Gangxiang Yuan, Yonggang Hu,Hongen Xu, Zhonghuai Xiang, Ze Zhang*. Evidence of selection at melanin synthesis pathway loci during silkworm domestication.Molecular Biology and Evolution, 2011, 28, 1785-1799.
