Multifunctional nanosheets based on hyaluronic acid modified graphene oxide for tumor-targeting chemophotothermal therapy.

【作者】 Lin Hou, Qianhua Feng,• Yating Wang,• Huijuan Zhang,•Guixiang Jiang, Xiaomin Yang ,• Junxiao Ren, Xiali Zhu,•Yuyang Shi , Zhenzhong Zhang

【期刊名】J Nanopart Res

【影响因子】 2015年: 2.101

【作者单位】 School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, PR China

【年，卷(期)：页码】2015,17:162

【关键词】Graphene oxide ,Hyaluronic acid ,Photothermal therapy , Chemotherapy , Cancer ,Nanomedicine

【摘要】Graphene oxide (GO) with strong optical absorption in the near-infrared (NIR) region has shown great potential both in photothermal therapy and drug delivery. In this work, hyaluronic acid (HA)-functionalized GO (HA-GO) was successfully synthesized and controlled loading of mitoxantrone (MIT) onto HA-GO via p–p stacking interaction was investigated. The results revealed that drug-loaded nanosheets with high loading efficiency of 45 wt% exhibited pH-sensitive responses to tumor environment.Owing to the receptor-mediated endocytosis, cellular uptake analysis of HA-GO showed enhanced internalization. In vivo optical imaging test demonstrated that HA-GO nanosheets could enhance the targeting ability and residence time in tumor site.Moreover, the anti-tumor activity of free MIT, MIT/GO, and MIT/HA-GO in combination with NIR laser was investigated using human MCF-7 cells. In vitro cytotoxicity study revealed that HA-GO could stand as a biocompatible nanocarrier and MIT/HA-GO demonstrated remarkably higher toxicity than free MIT and MIT/GO, with IC50 of 0.79 lg ml-1. Tumor cellkilling potency was enhanced when MIT/HA-GO were combined with NIR irradiation, and the IC50 of MIT/HA-GO plus laser irradiation was 0.38 lg ml-1.In vivo, MIT/HA-GO plus NIR laser irradiation with the tumor growth inhibition of 93.52 % displayed greater anti-tumor effect compared with free MIT and MIT/GO with or without laser irradiation. Therefore,the MIT/HA-GO nanosheets may potentially be useful for further development of synergistic cancer therapy.

【全文链接】https://link.springer.com/article/10.1007%2Fs11051-015-2966-9