A Probiotic Spore-Based Oral Autonomous Nanoparticles Generator for Cancer Therapy

【作者】Qingling Song, CuixiaZheng, JiajiaJia, Hongjuan Zhao, QianhuaFeng, Hongling Zhang, Lei Wang,* Zhenzhong Zhang,* and Yun Zhang*

【期刊名】Advanced Materials

【影响因子】 2018年:25.809

【作者单位】School of Pharmaceutical Sciences, Zhengzhou University, 100 Kexue Avenue, Zhengzhou 450001, PR China

【年，卷(期)：页码】2019, e1903793

【关键词】autonomous nanoparticles generator, probiotic spores, intestinalmicroenvironment, multi-biological barriers

【摘要】Spores, the dormant life forms of probiotics, can germinate to metabolically active vegetative cells with the disintegration of their hydrophobic protein coat in the intestinal microenvironment, which provides the possibility for the formation of nanoparticles (NPs) in vivo. Inspired by the natural physiological process of spores, herein, an oral autonomous NPs generator is developed to overcome the spatially variable gastrointestinal tract environment and multi-biological barriers. Spores modified with deoxycholic acid(DA) and loaded with chemotherapeutic drugs (doxorubicin and sorafenib,DOX/SOR) serve as an autonomous production line of NPs, which canefficaciously protect the drugs passing through the rugged environment ofthe stomach and furthermore can be transported to the intestinal environment and colonized rapidly. Subsequently, the DOX/SOR/Spore-DA NPsare produced by the autonomous NPs generator in the intestinal regionsbased on the disintegrated hydrophobic protein and the hydrophilic DA, andthey can efficiently penetrate the epithelial cells via the bile acid pathway,increasing basolateral drug release. In vitro and in vivo studies confirm thatthis biological nanogenerator can autonomously produce substantial NPs inthe intestine, providing a promising strategy for cancer therapy.

【全文链接】

https://onlinelibrary.wiley.com/doi/full/10.1002/adma.201903793